Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide ...

We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product accumulation, ...

In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.

Discover the magic of compounding and why it's important for increasing wealth Robert Kelly is managing director of XTS Energy LLC, and has more than three decades of experience as a business ...