Most researchers know they need a pure protein. Few talk about how genuinely difficult it is to get one. A cell lysate contains thousands of proteins competing for the same space. Your target is in ...

Elastin-like polypeptides (ELPs) undergo a reversible, inverse phase transition. Below their transition temperature (T t), ELPs are soluble in water, but when the temperature is raised above T t, ...

A carefully designed ammonium sulfate precipitation will simplify extraction of proteins and is considered to be a gold standard among various precipitation methods. Therefore, optimization of ...

Affinity chromatography can be applied to a wide array of proteins and works by exploiting the binding properties between proteins and ligands. It involves passing a recombinant protein mixture over a ...